Journal
/
/
Application of an In vitro DNA Protection Assay to Visualize Stress Mediation Properties of the Dps Protein
Journal JoVE
Biologie
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Biologie
Application of an In vitro DNA Protection Assay to Visualize Stress Mediation Properties of the Dps Protein
DOI:

12:41 min

May 31, 2013

, ,

Chapitres

  • 00:05Titre
  • 01:43Dps Expression and Purification
  • 06:46DNA Protection Assay
  • 09:42Results: Purified Dps Protects DNA from Oxidative Degradation
  • 11:58Conclusion

Summary

Traduction automatique

The DNA-binding protein from starved cells (Dps) plays a crucial role in combating bacterial stress. This article discusses the purification of E. coli Dps and the protocol for an in vitro assay demonstrating Dps-mediated protection of DNA from degradation by reactive oxygen species.

Vidéos Connexes

Read Article