Journal
/
Biologie
/
Imaging and 3D Reconstruction of Cerebrovascular Structures in Embryonic Zebrafish
Journal JoVE
Biologie
This content is Free Access.
Journal JoVE Biologie
Imaging and 3D Reconstruction of Cerebrovascular Structures in Embryonic Zebrafish

Imaging and 3D Reconstruction of Cerebrovascular Structures in Embryonic Zebrafish

DOI:
10.3791/50417-v

•

08:00 min

•

April 22, 2014

•

,
1Molecular Neurobiology,Western University of Health Sciences, 2Graduate College of Biomedical Sciences,Western University of Health Sciences, 3College of Osteopathic Medicine of the Pacific,Western University of Health Sciences, 4College of Optometry,Western University of Health Sciences

Chapitres

  • 00:05Titre
  • 01:23Zebrafish Husbandry, Embryo Generation, and Treatment
  • 02:50Confocal Imaging of Cerebrovascular Structures in Fixed Zebrafish Embryos
  • 04:073D Reconstruction of Embryonic Zebrafish Cerebrovasculature
  • 06:34Results: 3D Reconstruction of Embryonic Zebrafish Cerebrovasculature
  • 07:26Conclusion

Summary

Traduction automatique

Traduction automatique

Imaging of cerebrovascular development in larval zebrafish is described. Techniques to facilitate 3D imaging and modify cerebrovascular development using chemical treatments are also provided.

Tags

Keywords: Zebrafish3D ReconstructionCerebrovascular StructuresEmbryonicImagingConfocal MicroscopyEGFPVascular Endothelial CellsBrainRetinaBiologie du développementPathologyIn Vivo

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/50417?article_type=v

Live Imaging of the Zebrafish Embryonic Brain by Confocal Microscopy
check_url/fr/50417?article_type=v

Tissue Targeted Embryonic Chimeras: Zebrafish Gastrula Cell Transplantation
check_url/fr/50417?article_type=v

Microdissection of Zebrafish Embryonic Eye Tissues
check_url/fr/50417?article_type=v

Immunostaining of Dissected Zebrafish Embryonic Heart
check_url/fr/50417?article_type=v

A System for ex vivo Culturing of Embryonic Pancreas
check_url/fr/50417?article_type=v

Laser-inflicted Injury of Zebrafish Embryonic Skeletal Muscle
check_url/fr/50417?article_type=v

Analysis of Embryonic and Larval Zebrafish Skeletal Myofibers from Dissociated Preparations
check_url/fr/50417?article_type=v

Intravital Microscopy for Imaging Subcellular Structures in Live Mice Expressing Fluorescent Proteins
check_url/fr/50417?article_type=v

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
check_url/fr/50417?article_type=v

Live Cell Imaging and 3D Analysis of Angiotensin Receptor Type 1a Trafficking in Transfected Human Embryonic Kidney Cells Using Confocal Microscopy

Read Article