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Neurosciences

免疫组化分析大鼠中枢神经系统和周围淋巴结组织切片

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Neurosciences

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Journal JoVE Neurosciences

Immunohistochemical Analysis in the Rat Central Nervous System and Peripheral Lymph Node Tissue Sections

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免疫组化分析大鼠中枢神经系统和周围淋巴结组织切片

DOI:

10.3791/50425-v

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09:11 min

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November 14, 2016

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Milena Z. Adzemovic², Manuel Zeitelhofer³, Marianne Leisser, Ulricke Köck, Angela Kury, Tomas Olsson

¹Department of Clinical Neuroscience, Neuroimmunology Unit, Center for Molecular Medicine,Karolinska Institutet, ²Department of Neuroimmunology, Center for Brain Research,Medical University of Vienna, ³Department of Medical Biochemistry and Biophysics, Vascular Biology Unit,Karolinska Institutet

Chapitres

00:05Titre
01:46Tissue Preparation: Fixation, Dehydration and Embedding
02:50Tissue Sectioning, Mounting and Deparaffinization
04:32Antigen Retrieval, Blocking, and Incubation with the Primary Antibodies
06:38Visualization of the Bound Biotinylated Secondary Antibody
07:48Results: Double Immunostaining in the Rat Lymph Nodes and the Central Nervous System
08:42Conclusion

Summary

Traduction automatique

We here present an optimized, detailed protocol for double immunostaining in formalin-fixed, paraffin-embedded rat central nervous system (CNS) and peripheral lymph node (LN) tissue sections.

免疫组化分析大鼠中枢神经系统和周围淋巴结组织切片

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