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Produktion af<em> Xenopus tropicalis</em> Egg uddragene Identificer mikrotubuli-associerede RNA
Journal JoVE
Biologie
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Journal JoVE Biologie
Production of Xenopus tropicalis Egg Extracts to Identify Microtubule-associated RNAs

Produktion af<em> Xenopus tropicalis</em> Egg uddragene Identificer mikrotubuli-associerede RNA

DOI:
10.3791/50434-v

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10:01 min

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June 27, 2013

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1Department of Molecular Biology,Massachusetts General Hospital, 2Department of Genetics,Harvard Medical School

Chapitres

  • 00:05Titre
  • 01:15Generation of X. tropicalis Eggs
  • 03:24Preparation of Extract from X. tropicalis Eggs
  • 06:45Purification of Taxol-stabilized Microtubules from X. tropicalis Extract
  • 08:25Representative X. Tropicalis Extraction Results
  • 09:37Conclusion

Summary

Traduction automatique

Traduction automatique

Vi beskriver indsamlingen af ​​ubefrugtede<em> Xenopus tropicalis</em> Æg og produktion af en meiose II anholdt æggeekstrakt. Denne æggeekstrakt kan anvendes til at oprense mikrotubuli og mikrotubulus-associerede RNA'er.

Tags

Xenopus TropicalisEgg ExtractsMicrotubule-associated RNAsTranscript IdentificationHigh-throughput SequencingSubcellular StructuresRNA LocalizationXenopus Laevis

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