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Mechanical Stimulation-induced Calcium Wave Propagation in Cell Monolayers: The Example of Bovine Corneal Endothelial Cells
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Journal JoVE Biologie
Mechanical Stimulation-induced Calcium Wave Propagation in Cell Monolayers: The Example of Bovine Corneal Endothelial Cells

Mechanical Stimulation-induced Calcium Wave Propagation in Cell Monolayers: The Example of Bovine Corneal Endothelial Cells

DOI:
10.3791/50443-v

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10:46 min

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July 16, 2013

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, ,
1Department of Cellular and Molecular Medicine, Laboratory of Molecular and Cellular Signaling,KU Leuven

Chapitres

  • 00:05Titre
  • 02:38Isolation of Corneal Endothelial Cells
  • 04:56Culture of Bovine Corneal Endothelial Cells
  • 05:56Mechanical Stimulation for Inducing Calcium Wave
  • 07:44Results: Calcium Wave Propagation in Bovine Corneal Endothelial Cells
  • 09:39Conclusion

Summary

Traduction automatique

Traduction automatique

Intercellular Ca2+-waves are driven by gap junction channels and hemichannels. Here, we describe a method to measure intercellular Ca2+-waves in cell monolayers in response to a local single-cell mechanical stimulus and its application to investigate the properties and regulation of gap junction channels and hemichannels.

Tags

Intercellular CommunicationCalcium Wave PropagationMechanical StimulationCorneal Endothelial CellsGap Junction ChannelsHemichannelsATP ReleaseCx43Fluo4-AMMicromanipulator

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