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4D Multimodality Imaging of Citrobacter rodentium Infections in Mice
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Chapitres
- 00:05Titre
- 01:21Preparing the Bacteria
- 02:44Infection of Mice with Bioluminescent C. rodentium
- 04:09Daily Composite 3D Diffuse Light Imaging Tomography with Integrated μCT Imaging (DLIT-μCT) of Infected Mice
- 06:153D Reconstruction of DLIT-μCT Imaging Data
- 09:29Generation of a 4D Movie of C. rodentium Infection
- 10:24Monitored Infection with 5 x 109 cfu C. rodentium, Strain ICC180
- 12:16Conclusion
Multi-modality imaging is a valuable approach for studying bacterial colonization in small animal models. This protocol outlines infection of mice with bioluminescent Citrobacter rodentium and the longitudinal monitoring of bacterial colonization using composite 3D diffuse light imaging tomography with μCT imaging to create a 4D movie of C. rodentium infection.
