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The In ovo CAM-assay as a Xenograft Model for Sarcoma
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Médecine
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Journal JoVE Médecine
The In ovo CAM-assay as a Xenograft Model for Sarcoma

The In ovo CAM-assay as a Xenograft Model for Sarcoma

DOI:
10.3791/50522-v

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12:44 min

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July 17, 2013

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, , , , , ,
1Department of Orthopaedic Surgery and Traumatology,Ghent University Hospital, 2Department of Radiation Oncology and Experimental Cancer Research,Ghent University, 3Department of Virology, Parasitology, and Immunology,Ghent University, 4Pathlicon

Chapitres

  • 00:05Titre
  • 02:19Preparation of Tumor-derived Single Cell Suspensions
  • 03:59Opening of the Eggs
  • 05:21Inoculation Procedure
  • 06:23Imaging and Harvesting of the CAM
  • 07:20Results: Evaluation of the CAM
  • 11:30Conclusion

Summary

Traduction automatique

Traduction automatique

The in ovo chorioallantoic membrane (CAM) is grafted with fresh sarcoma-derived tumor tissues, their single cell suspensions, and permanent and transient fluorescently labeled established sarcoma cell lines. The model is used to study graft- (viability, Ki67 proliferation index, necrosis, infiltration) and host (fibroblast infiltration, vascular ingrowth) behavior.

Tags

Keywords: SarcomaXenograft ModelChick Chorioallantoic Membrane (CAM)In OvoCell LinesSaos-2SW1353Fluorescent LabelingGraft BehaviorHost ResponseKi67 Proliferation IndexNecrosisVascular IngrowthECM Gel

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