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Methods for Performing Crosses in Setaria viridis, a New Model System for the Grasses
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Chapitres
- 00:05Titre
- 01:10Growth of S. viridis
- 02:11Trimming and Labeling of Panicle Before Emasculation
- 03:49Emasculation with Warm Water-Dipping
- 04:47Crossing/Pollination After Emasculation and Seed Harvesting
- 07:32Results: Determination of Successfully Outcrossed Progeny
- 08:06Conclusion
We have developed a methodology for performing crosses in Setaria viridis (S. viridis). The method involves pruning the panicle prior to a hot water treatment to kill viable pollen. Crosses are performed following a well-controlled growth regime and typically result in the recovery of 1 to 7 cross-pollinated seed/s per panicle.
