Journal
/
Biologie
/
Lev Imaging Assay for at vurdere de roller Ca<sup> 2 +</sup> Og sphingomyelinase i reparation af poredannende Toxin Wounds
Journal JoVE
Biologie
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Biologie
Live Imaging Assay for Assessing the Roles of Ca2+ and Sphingomyelinase in the Repair of Pore-forming Toxin Wounds

Lev Imaging Assay for at vurdere de roller Ca<sup> 2 +</sup> Og sphingomyelinase i reparation af poredannende Toxin Wounds

DOI:
10.3791/50531-v

•

18:25 min

•

August 25, 2013

•

, ,
1Department of Cell Biology and Molecular Genetics,University of Maryland

Chapitres

  • 00:05Titre
  • 01:41Transcriptional Silencing of ASM
  • 04:18Preparation of Reagents for Live Microscopy
  • 05:29Live Cell Imaging of FM1-43 Influx into SLO Treated and Untreated Cells
  • 10:21Quantification of FM1-43 Influx into Cells
  • 14:03Results: Ca2+ and Sphingomyelinase are Involved in Plasma Membrane Repair
  • 17:34Conclusion

Summary

Traduction automatique

Traduction automatique

Levende billeddannelse af celler udsat for den lipofile farvestof FM1-43 muliggør en præcis bestemmelse af kinetikken, som poredannende toksiner er fjernet fra plasmamembranen. Det er et følsomt assay, der kan bruges til at vurdere kravene til Ca 2 +, sphingomyelinase og andre faktorer på plasma membran reparation.

Tags

Live ImagingCa2+SphingomyelinasePore-forming ToxinPlasma Membrane RepairFM1-43Streptolysin OExocytosisEndocytosis

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/50531?article_type=v

The Preparation of Drosophila Embryos for Live-Imaging Using the Hanging Drop Protocol
check_url/fr/50531?article_type=v

Live Imaging of the Zebrafish Embryonic Brain by Confocal Microscopy
check_url/fr/50531?article_type=v

Techniques for Imaging Ca2+ Signaling in Human Sperm
check_url/fr/50531?article_type=v

Electron Spin Resonance Micro-imaging of Live Species for Oxygen Mapping
check_url/fr/50531?article_type=v

Clonogenic Assay: Adherent Cells
check_url/fr/50531?article_type=v

En Eksplantation Assay for vurdering Cellulær Behavior af Cranial mesenkym
check_url/fr/50531?article_type=v

Levende Imaging af Apoptotisk Cell Clearance under<em> Drosophila</em> Embryogenese
check_url/fr/50531?article_type=v

Levende Cell Imaging af Early Autophagy begivenheder: Omegasomes and Beyond
check_url/fr/50531?article_type=v

Tomat / GFP-FLP / FRT Metode til Live Imaging af Mosaic Voksen<em> Drosophila</em> fotoreceptorceller
check_url/fr/50531?article_type=v

Adipo-klar: En væv Clearing metode til tredimensional billeddannelse af fedtvæv

Read Article