Journal
/
Biologie
/
Robust 3D DNA FISH Using Directly Labeled Probes
Journal JoVE
Biologie
This content is Free Access.
Journal JoVE Biologie
Robust 3D DNA FISH Using Directly Labeled Probes

Robust 3D DNA FISH Using Directly Labeled Probes

DOI:
10.3791/50587-v

•

12:16 min

•

August 15, 2013

•

, , , ,
1Nuclear Dynamics Programme,The Babraham Institute, 2Epigenetics Programme,The Babraham Institute, 3Centre for Trophoblast Research,University of Cambridge

Chapitres

  • 00:05Titre
  • 01:18Generating Directly Labeled DNA Probes by Nick Translation
  • 02:21Day 1: Probe Precipitation
  • 04:04Day 1: Treatment of the Cells
  • 08:03Day 2: Final Washes and Mounting
  • 09:24FISH Probe Quality Control and Application
  • 11:33Conclusion

Summary

Traduction automatique

Traduction automatique

We describe a robust and versatile protocol for analyzing nuclear architecture by 3D DNA FISH using directly labeled fluorescent probes.

Tags

Keywords: 3D DNA FISHDirectly Labeled ProbesNick-translationFreeze-thaw PermeabilizationNuclear OrganizationChromosomal RegionsHigh-throughput Imaging

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/50587?article_type=v

Direct Observation of Enzymes Replicating DNA Using a Single-molecule DNA Stretching Assay
check_url/fr/50587?article_type=v

DNA-based Fish Species Identification Protocol
check_url/fr/50587?article_type=v

Combined Immunofluorescence and DNA FISH on 3D-preserved Interphase Nuclei to Study Changes in 3D Nuclear Organization
check_url/fr/50587?article_type=v

Assessing Species-specific Contributions To Craniofacial Development Using Quail-duck Chimeras
check_url/fr/50587?article_type=v

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons
check_url/fr/50587?article_type=v

Combined DNA-RNA Fluorescent In situ Hybridization (FISH) to Study X Chromosome Inactivation in Differentiated Female Mouse Embryonic Stem Cells
check_url/fr/50587?article_type=v

Amplification, Next-generation Sequencing, and Genomic DNA Mapping of Retroviral Integration Sites
check_url/fr/50587?article_type=v

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes
check_url/fr/50587?article_type=v

Observation and Quantification of Telomere and Repetitive Sequences Using Fluorescence In Situ Hybridization (FISH) with PNA Probes in Caenorhabditis elegans
check_url/fr/50587?article_type=v

Fish Sperm Assessment Using Software and Cooling Devices

Read Article