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Proteomiske Prøveforberedelse fra formalin Faste og Paraffin Embedded Tissue
Journal JoVE
Biologie
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Journal JoVE Biologie
Proteomic Sample Preparation from Formalin Fixed and Paraffin Embedded Tissue

Proteomiske Prøveforberedelse fra formalin Faste og Paraffin Embedded Tissue

DOI:
10.3791/50589-v

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09:20 min

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September 02, 2013

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1Department of Proteomics and Signal Transduction,Max Planck Institute of Biochemistry

Chapitres

  • 00:05Titre
  • 01:46Preparation of Tissue Slices for Microdissection and Sample Lysis
  • 03:20Sample Processing
  • 04:51Quantification of the Peptides
  • 05:38Fractionation of the Lys-C and Tryptic Peptides
  • 07:29Results: Peptide Quantification
  • 08:21Conclusion

Summary

Traduction automatique

Traduction automatique

Archival formalin faste og paraffinindlejret (FFPE) kliniske prøver er værdifuldt materiale til undersøgelse af sygdomme. Her demonstrerer vi en prøveforberedelse workflow tillader indgående proteomisk analyse af mikrodissekeres FFPE væv.

Tags

Keywords: ProteomicSample PreparationFASPLysCTrypsinPeptide FractionationQuantitative AnalysisBiomarkersDrug Targets

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