/
/
A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments
Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Chapitres
- 00:05Titre
- 01:59Assembly and Population of the Cell Perfusion Chambers
- 04:28Fabrication of the Digital Microfluidic (DMF) Module and Assembly of the Hub Architecture
- 06:37Stimulation of Cell Populations
- 07:45Terminate Stimulation and Recovery of Cell Lysates
- 09:19Results: Micro-scale Cell Stimulation Produces Results Similar to Conventional Cultures
- 11:16Conclusion
We have developed an automated cell culture and interrogation platform for micro-scale cell stimulation experiments. The platform offers simple, versatile, and precise control in cultivating and stimulating small populations of cells, and recovering lysates for molecular analyses. The platform is well suited to studies that use precious cells and/or reagents.
