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Immunologie et infection
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Genetic Manipulation in Δku80 Strains for Functional Genomic Analysis of Toxoplasma gondii
Journal JoVE
Immunologie et infection
This content is Free Access.
Journal JoVE Immunologie et infection
Genetic Manipulation in Δku80 Strains for Functional Genomic Analysis of Toxoplasma gondii

Genetic Manipulation in Δku80 Strains for Functional Genomic Analysis of Toxoplasma gondii

DOI:
10.3791/50598-v

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09:52 min

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July 12, 2013

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, , , , , ,
1Department of Microbiology and Immunology,The Geisel School of Medicine at Dartmouth

Chapitres

  • 00:05Titre
  • 01:58Preparation of Targeting DNA Plasmid pGOI
  • 02:48Transfecting T. gondii Parasites
  • 03:43Selecting Transfected T. gondii Parasites
  • 07:07Deletion of HXGPRT Marker and C-terminal Tagging
  • 07:48Results: Deletion of rop18 and Removal of the HXGPRT Selectable Marker from gra2
  • 08:54Conclusion

Summary

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Here we report a method for using type I and type II Δku80 strains of Toxoplasma gondii to efficiently generate targeted gene deletions and gene replacements for functional genomic analysis.

Tags

Genetic ManipulationΔKU80 StrainsToxoplasma GondiiFunctional Genomic AnalysisHomologous RecombinationGene DeletionGene ReplacementGene TaggingHXGPRT Selectable Marker

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