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Stereotaxic Microinjection of Viral Vectors Expressing Cre Recombinase to Study the Role of Target Genes in Cocaine Conditioned Place Preference
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Sciences du comportement
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Journal JoVE Sciences du comportement
Stereotaxic Microinjection of Viral Vectors Expressing Cre Recombinase to Study the Role of Target Genes in Cocaine Conditioned Place Preference

Stereotaxic Microinjection of Viral Vectors Expressing Cre Recombinase to Study the Role of Target Genes in Cocaine Conditioned Place Preference

DOI:
10.3791/50600-v

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08:22 min

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July 30, 2013

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,
1Graduate Program in Neuroscience,Weill Cornell Graduate School of Biomedical Sciences, 2Division of Pediatric Neurology, Department of Pediatrics,Weill Cornell Medical College

Chapitres

  • 00:05Titre
  • 01:36Preparation and Setup for Stereotaxic Delivery of Viral Vectors
  • 02:32Stereotaxic Microinjection of Viral Vectors
  • 04:41Conditioned Place Reference: Acquisition, Extinction, and Reinstatement
  • 05:55Representative Cocaine Conditioned Place Preference Results
  • 07:39Conclusion

Summary

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Traduction automatique

This article describes how to microinject viral vectors into mouse brain and then test in a conditioned place preference paradigm that includes an acquisition, extinction and reinstatement phase.

Tags

Keywords: Stereotaxic MicroinjectionViral VectorsCre RecombinaseConditional Gene KnockoutCocaineConditioned Place PreferenceAddictionBehavioral ModelsTemporally-specificRegionally-specificGene DeletionGene TargetingRAAVCocaine-induced PlasticityWithdrawalExtinctionReinstatement

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