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Isolation of Human Hepatocytes by a Two-step Collagenase Perfusion Procedure
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Journal JoVE Biologie
Isolation of Human Hepatocytes by a Two-step Collagenase Perfusion Procedure

Isolation of Human Hepatocytes by a Two-step Collagenase Perfusion Procedure

DOI:
10.3791/50615-v

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07:09 min

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September 03, 2013

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, , , ,
1Experimental Surgical Research,Grosshadern Hospital, Munich, 2Center for Liver Cell Research,Grosshadern Hospital, Munich, 3Hepacult LLC, Regensburg, 4Department of Surgery,Grosshadern Hospital, Munich

Chapitres

  • 00:05Titre
  • 01:26Preparation of Perfusion Equipment and Solutions
  • 02:00Perfusion of the Liver
  • 04:29Isolation of Hepatocytes
  • 06:03Isolated Hepatocyte Viability, Yield and Purity
  • 06:47Conclusion

Summary

Traduction automatique

Traduction automatique

A modified two-step collagenase perfusion procedure for isolation of human hepatocytes is described. This method can also be applied to other mammalian livers. The isolated hepatocytes are available in high yield and viability, making them a suitable model for scientific research in areas such as liver regeneration, pharmacokinetics and toxicology.

Tags

HepatocytesLiverCollagenase PerfusionIsolationTwo-stepHumanRegenerationPharmacokineticsToxicologyTranslational ResearchEnzymatic MethodStructural IntegrityFunction

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