Journal
/
Bio-ingénierie
/
A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
Journal JoVE
Bio-ingénierie
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Bio-ingénierie
A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

DOI:
10.3791/50618-v

•

15:41 min

•

October 15, 2013

•

, , ,
1Department of Chemistry and Applied Biosciences,ETH Zurich, Switzerland

Chapitres

  • 00:05Titre
  • 02:13Fabrication of the Microfluidic Chip
  • 05:31Chip Preparation for Enzymatic Assays
  • 07:15Additional Chip Preparation for Immunoassays
  • 10:05Cell Harvesting and Glucose 6-Phosphate Dehydrogenase Assay
  • 12:08Results: Enzymatic and Antibody Analysis of Single Cells
  • 15:08Conclusion

Summary

Traduction automatique

Traduction automatique

In this article we present a microfluidic chip for single cell analysis. It allows the quantification of intracellular proteins, enzymes, cofactors, and second messengers by means of fluorescent assays or immunoassays. 

Tags

Microfluidic ChipSingle-cell AnalysisIntracellular BiomoleculesQuantitative DeterminationCell IsolationCell LysisLysate RetentionHigh SensitivityArray FormatAnalysis Of ProteinsEnzymesCofactorsSecond Messengers

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

Microfluidic Device for Recreating a Tumor Microenvironment in Vitro

A Microfluidic-based Hydrodynamic Trap for Single Particles

Fluorescence detection methods for microfluidic droplet platforms

Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis: Fabrication, System Setup, and Operation

A Microfluidic Chip for ICPMS Sample Introduction

The Multi-organ Chip – A Microfluidic Platform for Long-term Multi-tissue Coculture

Electrotaxis Studies of Lung Cancer Cells using a Multichannel Dual-electric-field Microfluidic Chip

Co-culture of Living Microbiome with Microengineered Human Intestinal Villi in a Gut-on-a-Chip Microfluidic Device

Designing Microfluidic Devices for Studying Cellular Responses Under Single or Coexisting Chemical/Electrical/Shear Stress Stimuli

A Versatile Method of Patterning Proteins and Cells

Read Article