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En Mikrofluid Chip til Alsidig kemisk analyse af enkelte celler
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Bio-ingénierie
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Journal JoVE Bio-ingénierie
A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

En Mikrofluid Chip til Alsidig kemisk analyse af enkelte celler

DOI:
10.3791/50618-v

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15:41 min

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October 15, 2013

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, , ,
1Department of Chemistry and Applied Biosciences,ETH Zurich, Switzerland

Chapitres

  • 00:05Titre
  • 02:13Fabrication of the Microfluidic Chip
  • 05:31Chip Preparation for Enzymatic Assays
  • 07:15Additional Chip Preparation for Immunoassays
  • 10:05Cell Harvesting and Glucose 6-Phosphate Dehydrogenase Assay
  • 12:08Results: Enzymatic and Antibody Analysis of Single Cells
  • 15:08Conclusion

Summary

Traduction automatique

Traduction automatique

I denne artikel præsenterer vi en mikrofluid chip til enkelt celle analyse. Det giver mulighed for kvantificering af intracellulære proteiner, enzymer, cofaktorer, og sekundære budbringere ved hjælp af fluorescerende eller immunassays.

Tags

Microfluidic ChipSingle-cell AnalysisIntracellular BiomoleculesQuantitative DeterminationCell IsolationCell LysisLysate RetentionHigh SensitivityArray FormatAnalysis Of ProteinsEnzymesCofactorsSecond Messengers

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