Journal
/
Biologie
/
Temporal Kvantificering af MAPK induceret ekspression i Single gærceller
Journal JoVE
Biologie
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Biologie
Temporal Quantification of MAPK Induced Expression in Single Yeast Cells

Temporal Kvantificering af MAPK induceret ekspression i Single gærceller

DOI:
10.3791/50637-v

•

07:59 min

•

October 04, 2013

•

, ,
1Department of Fundamental Microbiology,University of Lausanne

Chapitres

  • 00:05Titre
  • 01:25Microscopy Measurements
  • 03:20Flow Cytometry Measurements
  • 05:02Results: Representative Microscopy and Flow Cytometry Measurement Data
  • 07:26Conclusion

Summary

Traduction automatique

Traduction automatique

To komplementære metoder baseret på flowcytometri og mikroskopi præsenteres som muligt at kvantificere, på enkelt celle niveau, af dynamikken i genekspression induceret ved aktivering af en MAPK-vejen i gær.

Tags

Gene ExpressionSingle CellMAPK PathwayYeastFlow CytometryMicroscopyFluorescent ReporterProtein ExpressionLive Cell Imaging

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/50637?article_type=v

Vaccinia Virus Infection & Temporal Analysis of Virus Gene Expression: Part 1
check_url/fr/50637?article_type=v

Vaccinia Virus Infection & Temporal Analysis of Virus Gene Expression: Part 2
check_url/fr/50637?article_type=v

Vaccinia Virus Infection & Temporal Analysis of Virus Gene Expression: Part 3
check_url/fr/50637?article_type=v

Using the Gene Pulser MXcell Electroporation System to Transfect Primary Cells with High Efficiency
check_url/fr/50637?article_type=v

Expression of Recombinant Proteins in the Methylotrophic Yeast Pichia pastoris
check_url/fr/50637?article_type=v

Identification of Growth Inhibition Phenotypes Induced by Expression of Bacterial Type III Effectors in Yeast
check_url/fr/50637?article_type=v

Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells
check_url/fr/50637?article_type=v

Analyse af Global RNA-syntese på enkelt celle niveau efter Hypoxia
check_url/fr/50637?article_type=v

Imaging Rumlig Reorganisering af et MAPK signalvej Brug af tobak Transient Expression System
check_url/fr/50637?article_type=v

Brug mikrofluidenheder til måling Levetid og cellefænotyper i Single Spirende gærceller

Read Article