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细胞和生物膜细胞外成分的定量并发
Journal JoVE
Immunologie et infection
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Journal JoVE Immunologie et infection
Concurrent Quantification of Cellular and Extracellular Components of Biofilms
DOI:

10:18 min

December 10, 2013

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Chapitres

  • 00:05Titre
  • 01:45Specimen Fabrication
  • 02:23Biofilm Growth
  • 04:00Antibacterial/Mouthwash Treatments
  • 04:33Staining
  • 05:36Imaging Using Confocal Laser Scanning Microscopy
  • 06:55Biofilm Visualization and Structural Analysis
  • 07:56Results: Representative Biofilm Component Staining for Mouthwash Treatment and Control Groups
  • 09:52Conclusion

Summary

Traduction automatique

A protocol for the concurrent quantification and comparison of three cellular and extracellular components within biofilms is presented. The methodology involves the use of confocal laser scanning microscopy, biofilm structural analysis and visualization software, and statistical analysis software.

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