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High Resolution Hele Mount<em> In Situ</em> Hybridisering inden Zebrafisk embryoner at studere genekspression og funktion
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Journal JoVE Biologie
High Resolution Whole Mount In Situ Hybridization within Zebrafish Embryos to Study Gene Expression and Function

High Resolution Hele Mount<em> In Situ</em> Hybridisering inden Zebrafisk embryoner at studere genekspression og funktion

DOI:
10.3791/50644-v

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10:06 min

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October 19, 2013

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,
1Endocrine Research Laboratory and Department of Medicine,Royal Victoria Hospital, 2McGill University Health Centre Research Institute

Chapitres

  • 00:05Titre
  • 01:31Synthesis of Digoxygenin-labeled RNA Probes
  • 03:32Whole Mount in situ Hybridization
  • 06:55Representative in situ Hybridization Results
  • 09:39Conclusion

Summary

Traduction automatique

Traduction automatique

Den zebrafisk, en lille tropisk fisk, er blevet en populær model for at studere genfunktion under hvirveldyr udvikling og sygdom. Det tidsmæssige og rumlige ekspression af målgener kan bestemmes ved<em> In situ</em> Hybridisering. Vores forbedrede protokol giver mulighed for påvisning af lave rigelige transkripter med lav ikke-specifik baggrund signal.

Tags

Gene ExpressionWhole Mount In Situ HybridizationWISHZebrafish EmbryosAntisense RNA ProbesDigoxigeninIn Vitro TranscriptionChromogenic SubstrateAlkaline PhosphataseDevelopmental Stage

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