Journal
/
Biologie
/
Høy oppløsning Whole Mount<em> In Situ</em> Hybridisering innenfor sebrafisk embryoer å studere Gene Expression og funksjon
Journal JoVE
Biologie
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Biologie
High Resolution Whole Mount In Situ Hybridization within Zebrafish Embryos to Study Gene Expression and Function

Høy oppløsning Whole Mount<em> In Situ</em> Hybridisering innenfor sebrafisk embryoer å studere Gene Expression og funksjon

DOI:
10.3791/50644-v

•

10:06 min

•

October 19, 2013

•

,
1Endocrine Research Laboratory and Department of Medicine,Royal Victoria Hospital, 2McGill University Health Centre Research Institute

Chapitres

  • 00:05Titre
  • 01:31Synthesis of Digoxygenin-labeled RNA Probes
  • 03:32Whole Mount in situ Hybridization
  • 06:55Representative in situ Hybridization Results
  • 09:39Conclusion

Summary

Traduction automatique

Traduction automatique

Sebrafisk, en liten tropisk fisk, har blitt en populær modell for å studere gen-funksjon i løpet av virveldyr utvikling og sykdom. Den tidsmessige og romlige ekspresjon av målgener kan bestemmes ved<em> In situ</em> Hybridisering. Vår forbedrede Protokollen tillater påvisning av lave rikelig transkripter med lav ikke-spesifikk bakgrunn signal.

Tags

Gene ExpressionWhole Mount In Situ HybridizationWISHZebrafish EmbryosAntisense RNA ProbesDigoxigeninIn Vitro TranscriptionChromogenic SubstrateAlkaline PhosphataseDevelopmental Stage

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/50644?article_type=v

Double Whole Mount in situ Hybridization of Early Chick Embryos
check_url/fr/50644?article_type=v

Microinjection of Zebrafish Embryos to Analyze Gene Function
check_url/fr/50644?article_type=v

Zebrafish Whole Mount High-Resolution Double Fluorescent In Situ Hybridization
check_url/fr/50644?article_type=v

Using the Gene Pulser MXcell Electroporation System to Transfect Primary Cells with High Efficiency
check_url/fr/50644?article_type=v

Using Whole Mount in situ Hybridization to Link Molecular and Organismal Biology
check_url/fr/50644?article_type=v

Whole Mount in Situ Hybridization of E8.5 to E11.5 Mouse Embryos
check_url/fr/50644?article_type=v

Radioaktivt In situ Hybridisering for å oppdage Diverse Gene Expression Mønstre i Tissue
check_url/fr/50644?article_type=v

Hele Mount RNA Fluorescent<em> In situ</em> Hybridisering av<em> Drosophila</em> Embryoer
check_url/fr/50644?article_type=v

RNA<em> I situ</em> Hybridisering i Whole Mount embryoer og cellehistologi Tilrettelagt for Marine Elasmobranchs
check_url/fr/50644?article_type=v

Analysis of Apoptosis in Zebrafish Embryos by Whole-mount Immunofluorescence to Detect Activated Caspase 3

Read Article