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Storstilet Gene Knockdown i
 
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Storstilet Gene Knockdown i

Article DOI: 10.3791/50693-v • 18:38 min • September 25th, 2013
September 25th, 2013 •
1, 2, 3
1Molecular and Cellular Biology Program, University of Massachusetts, Amherst, 2Neuroscience and Behavior Program, University of Massachusetts, Amherst, 3Department of Biochemistry and Molecular Biology, University of Massachusetts, Amherst

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Mens dsRNA fodring i C. elegans er et kraftfuldt værktøj til at vurdere gen funktion, nuværende protokoller for storstilede fodring skærme resulterer i variable knockdown effektivitetsgevinster. Vi beskriver en forbedret protokol til at udføre storstilet RNAi fodring skærme, der resulterer i meget effektive og reproducerbar knockdown af genekspression.

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Developmental Biology Caenorhabditis elegans (C. elegans) Gene Knockdown Techniques, gen knockdown stor målestok fodring skærm
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