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Deriving the Time Course of Glutamate Clearance with a Deconvolution Analysis of Astrocytic Transporter Currents
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Neurosciences
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Journal JoVE Neurosciences
Deriving the Time Course of Glutamate Clearance with a Deconvolution Analysis of Astrocytic Transporter Currents

Deriving the Time Course of Glutamate Clearance with a Deconvolution Analysis of Astrocytic Transporter Currents

DOI:
10.3791/50708-v

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09:42 min

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August 07, 2013

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1Synaptic Physiology Section, National Institute of Neurological Disorders and Stroke,National Institutes of Health

Chapitres

  • 00:05Titre
  • 02:15Slice Preparation
  • 03:54Astrocyte Identification and Recordings
  • 04:46Isolation of the Facilitated Portion of Synaptically-activated Transporter Currents
  • 07:15Isolation of Flash-activated Transporter Currents
  • 07:58Results: Deconvolution Analysis
  • 08:58Conclusion

Summary

Traduction automatique

Traduction automatique

We describe an analytical method to estimate the lifetime of glutamate at astrocytic membranes from electrophysiological recordings of glutamate transporter currents in astrocytes.

Tags

Keywords: Glutamate ClearanceAstrocytic Transporter CurrentsGlutamate TransportersAstrocytesWhole-cell Patch-clamp RecordingsGlutamate Concentration ProfileTransporter KineticsElectrotonic PropertiesGlutamate UncagingSynaptic Glutamate ReleaseCentral Nervous SystemHealthDisease

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