/

/

由外显子捕获和大规模平行测序检测体细胞遗传学改变在肿瘤标本

Journal JoVE
Biologie

Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.

Journal JoVE Biologie

Detecting Somatic Genetic Alterations in Tumor Specimens by Exon Capture and Massively Parallel Sequencing

Please note that all translations are automatically generated. Click here for the English version.

由外显子捕获和大规模平行测序检测体细胞遗传学改变在肿瘤标本

DOI:

10.3791/50710-v

•

11:02 min

•

October 18, 2013

•

Helen H Won, Sasinya N Scott, A. Rose Brannon, Ronak H Shah, Michael F Berger²

¹Department of Pathology,Memorial Sloan-Kettering Cancer Center, ²Human Oncology and Pathogenesis Program,Memorial Sloan-Kettering Cancer Center

Chapitres

00:05Titre
02:08Library Preparation: End Repair
04:12Library Preparation: dA-Tailing and Adapter Ligation
05:55Roche Nimblegen SeqCAP EZ Library Hybridization, Wash, and Amplification
08:31Tumor-normal Pair Analysis
10:08Conclusion

Summary

Traduction automatique

我们描述条形码DNA文库和随后的杂交为基础的外显子捕获检测的关键癌症相关的基因突变在临床肿瘤标本通过大规模并行的“下一代”测序的准备。靶向外显子的测序提供了高吞吐量，低成本，以及深的序列覆盖率的好处，从而产生较高的灵敏度，用于检测低频率突变。

Tags

Somatic Genetic Alterations Tumor Specimens Exon Capture Massively Parallel Sequencing Next-generation Sequencing Cancer-associated Mutations Sequence Mutations Copy Number Alterations Structural Rearrangements Targeted Exon Sequencing High Throughput Low Cost Deep Sequence Coverage High Sensitivity

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

CD4 + T淋巴细胞艾滋病毒的捕获使用一次性微流体芯片

双整装原位杂交技术在早期鸡胚

In vivo Reprogramming of Adult Somatic Cells to Pluripotency by Overexpression of Yamanaka Factors

从人类体细胞与仙台病毒高效的新一代人类诱导多能干细胞

线性扩增介导的PCR - 未知侧翼DNA的遗传物质和表征本地化

在培养的哺乳动物细胞大规模并行报告基因分析

糖皮质激素粪分析：在马属动物无创监测肾上腺

有针对性的RNA测序分析表征基因表达和基因组改变

线粒体钙和线粒体膜的同时测量通过荧光显微镜活细胞的潜在

Interactome: Domainome 图书馆建设、噬菌体展示和下一代测序的验证与选择协议

Read Article