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Immunologie et infection
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Live Cell Imaging of Alphaherpes Virus Anterograde Transport and Spread
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Immunologie et infection
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Journal JoVE Immunologie et infection
Live Cell Imaging of Alphaherpes Virus Anterograde Transport and Spread

Live Cell Imaging of Alphaherpes Virus Anterograde Transport and Spread

DOI:
10.3791/50723-v

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15:31 min

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August 16, 2013

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, ,
1Department of Immunology and Infectious Diseases,Montana State University, 2Department of Molecular Biology,Princeton University

Chapitres

  • 00:05Titre
  • 01:27Environmental Control Conditions for Live-cell Fluorescent Microscopy
  • 03:44Real-time Imaging of Anterograde Virion Transport Events
  • 06:43Overnight Time-lapse Imaging of Anterograde Spread Events
  • 09:08Image Processing and Export
  • 10:48Results: Representative Live Cell Imaging of Alphaherpes Virus Anterograde Transport and Spread
  • 15:06Conclusion

Summary

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Traduction automatique

Live cell imaging of alphaherpes virus infections enables analysis of the dynamic events of directed transport and intercellular spread. Here, we present methodologies that utilize recombinant viral strains expressing fluorescent fusion proteins to facilitate visualization of viral assemblies during infection of primary neurons.

Tags

Anterograde TransportLive Cell ImagingFluorescent Fusion ProteinsViral Particle AssembliesCell-cell SpreadParticle Transport VelocitiesProtein LocalizationViral Genetic TechniquesAlphaherpes Virus

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