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Murine Cervical Heart Transplantation Model Using a Modified Cuff Technique
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Médecine
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Journal JoVE Médecine
Murine Cervical Heart Transplantation Model Using a Modified Cuff Technique

Murine Cervical Heart Transplantation Model Using a Modified Cuff Technique

DOI:
10.3791/50753-v

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10:32 min

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October 12, 2014

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, , , , , , , , , ,
1Center of Operative Medicine, Department of Visceral, Transplant and Thoracic Surgery,Innsbruck Medical University, 2Department of Plastic and Reconstructive Surgery,Johns Hopkins University School of Medicine

Chapitres

  • 00:00Titre
  • 00:07Introduction
  • 00:31Recipient Preparation
  • 05:06Heart Procurement
  • 07:39Implantation
  • 09:28Representative Results
  • 09:51Conclusion

Summary

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The murine cervical heart transplantation model is well suited for immunological as well as ischemia reperfusion injury studies. We modified the procedure using a non-suture cuff technique and performed more than 1,000 successful transplants with this approach.

Herein, we provide additional details of this technique to supplement the video.

Tags

MurineCervical Heart TransplantationCuff TechniqueMouse ModelsHeart TransplantationSuture TechniqueRatsNon-suture Cuff TechniquesVascular AnastomosisCervical Heart TransplantsCommon Carotid ArteryAortic ArchPulmonary ArteryExternal Jugular Vein

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