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发展多深的圆形横截面微内皮化的一个芯片的程序

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Journal JoVE Bio-ingénierie

Procedure for the Development of Multi-depth Circular Cross-sectional Endothelialized Microchannels-on-a-chip

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发展多深的圆形横截面微内皮化的一个芯片的程序

DOI:

10.3791/50771-v

•

10:55 min

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October 21, 2013

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Xiang Li, Samantha Marie Mearns, Manuela Martins-Green, Yuxin Liu

¹Lane Department of Computer Science and Electrical Engineering,West Virginia University, ²Department of Cell Biology and Neuroscience,University of California at Riverside

Chapitres

00:05Titre
02:22Photolithography Fabrication of Photoresist Master Mold
03:10Soft Lithography Fabrication of PDMS Microchannel Network
04:34HUVEC Culture and Seeding in the Chip
07:03Cell Staining and Microscope Characterization
08:10Results: Multi-depth Microchannel Networks Mimic the Complex 3D Geometries of In vivo Microvessel
09:35Conclusion

Summary

Traduction automatique

Â微通道上的一个芯片平台开发回流焊的光致抗蚀剂光刻技术，软光刻技术和微流体的结合。模拟的三维（3D）的几何形状在体内微血管内皮化的微通道平台，控制连续灌注流量下运行，允许高品质和实时成像，并可以应用对微血管研究。

Tags

Multi-depth Circular Cross-sectional Microchannels Endothelialized Microchannels In Vitro Microvessels 3D Geometry Continuous Fluid Flow Photolithographic Reflow Soft Lithography Microfluidics PDMS Primary Human Umbilical Vein Endothelial Cells (HUVECs)Perfusion

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