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Establishing the Minimal Bactericidal Concentration of an Antimicrobial Agent for Planktonic Cells (MBC-P) and Biofilm Cells (MBC-B)
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Immunologie et infection
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Journal JoVE Immunologie et infection
Establishing the Minimal Bactericidal Concentration of an Antimicrobial Agent for Planktonic Cells (MBC-P) and Biofilm Cells (MBC-B)

Establishing the Minimal Bactericidal Concentration of an Antimicrobial Agent for Planktonic Cells (MBC-P) and Biofilm Cells (MBC-B)

DOI:
10.3791/50854-v

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06:36 min

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January 02, 2014

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1Department of Biochemistry, Microbiology and Immunology,University of Ottawa

Chapitres

  • 00:05Titre
  • 01:03Minimal Bactericidal Concentration of Biofilm Cells (MBC-B)
  • 02:40Assay for Live Cells
  • 03:34Minimal Bactericidal Concentration of Planktonic Cells (MBC-P)
  • 05:22Results: MBC-P and MBC-B Assays
  • 06:18Conclusions

Summary

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Traduction automatique

This protocol allows for a direct comparison between planktonic and biofilm resistance for a bacterial strain that can form a biofilm in vitro using a 96-well microtiter plate. Planktonic or biofilm bacteria are exposed to serial dilutions of the antimicrobial agent of choice. Viability is assayed by growth on agar plates.

Tags

Keywords: Minimal Bactericidal ConcentrationMBCAntimicrobial AgentPlanktonic CellsMBC-PBiofilm CellsMBC-BBacterial StrainMicrotiter PlateAntimicrobial ResistanceBiofilm FormationBiofilm RecoveryCell Viability Assay

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