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Membrane Potential Dye Imaging of Ventromedial Hypothalamus Neurons From Adult Mice to Study Glucose Sensing
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Journal JoVE Neurosciences
Membrane Potential Dye Imaging of Ventromedial Hypothalamus Neurons From Adult Mice to Study Glucose Sensing

Membrane Potential Dye Imaging of Ventromedial Hypothalamus Neurons From Adult Mice to Study Glucose Sensing

DOI:
10.3791/50861-v

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11:10 min

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November 27, 2013

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, ,
1Department of Pharmacology and Physiology,Rutgers New Jersey Medical School, 2Center for Taste and Feeding Behavior,Universite de Bourgogne

Chapitres

  • 00:05Titre
  • 01:18Brain Slicing and Dissection
  • 02:35Dissociation and Culture
  • 05:50Preparation for Membrane Potential Dye Imaging
  • 08:23MPD Imaging
  • 09:36Results: Membrane Potential Dye Imaging of Ventromedial Hypothalamus Neurons
  • 10:43Conclusion

Summary

Traduction automatique

Traduction automatique

The activity of single neurons from adult-aged mice can be studied by dissociating neurons from specific brain regions and using fluorescent membrane potential dye imaging. By testing responses to changes in glucose, this technique can be used to study the glucose sensitivity of adult ventromedial hypothalamic neurons.

Tags

Membrane Potential DyeVentromedial HypothalamusGlucose SensingAdult MiceFluorescence ImagingNeuronal ActivityElectrophysiologyHeterogeneous Neuronal PopulationEnergy BalanceObesity

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