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Systematisk analyse af<em> In Vitro</em> Cell Rolling Ved hjælp af en multi-brønds plade Mikrofluid System
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1Division of Biomedical Engineering, Department of Medicine,Brigham and Women’s Hospital, 2Center for Regenerative Therapeutics,Brigham and Women’s Hospital, 3Harvard Medical School,Harvard University, 4Harvard Stem Cell Institute,Harvard University, 5Harvard-MIT Division of Health Sciences and Technology, 6Department of Mechanical Engineering,Massachusetts Institute of Technology
Chapitres
- 00:05Titre
- 01:24Coating of Microfluidic Channels with Fibronectin, P-selectin, or E-selectin
- 02:07Creation of a Chinese Hamster Ovary P-selectin (CHO-P) or Lung Microvascular Endothelial Cells (LMVEC) Monolayer Inside the Microfluidic Channel
- 03:22LMVEC Pro-inflammatory Activation and Antibody Blocking of P/E-selectin
- 04:09HL-60 Rolling Assay on Substrate/Cell Monolayer-coated Microfluidic Channels
- 05:41Results: Analysis of HL-60 Rolling Using the MIcrofluidic Channel System
- 10:22Conclusion
Denne undersøgelse bruges en multi-brønds plade microfluidic system markant stigende gennemløb celle rullende studier under fysiologisk relevant shear flow. I betragtning af betydningen af celle rullende i multi-step celle målsøgende kaskade og betydningen af celle målsøgende efter systemisk levering af udefra kommende populationer af celler i patienter, giver dette system potentiale som en screening platform til at forbedre celle-baseret behandling.
