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The Cell-based L-Glutathione Protection Assays to Study Endocytosis and Recycling of Plasma Membrane Proteins
Journal JoVE
Biologie
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Journal JoVE Biologie
The Cell-based L-Glutathione Protection Assays to Study Endocytosis and Recycling of Plasma Membrane Proteins

The Cell-based L-Glutathione Protection Assays to Study Endocytosis and Recycling of Plasma Membrane Proteins

DOI:
10.3791/50867-v

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09:22 min

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December 13, 2013

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1Department of Nephrology,Children’s Hospital of Pittsburgh of UPMC, 2Department of Cell Biology and Physiology,University of Pittsburgh School of Medicine

Chapitres

  • 00:05Titre
  • 01:45Endocytic Assay: Biotinylation of the Apical Membrane Domain
  • 07:54Results: CFTR Endocytosis in CFBE41o- Cells
  • 08:49Conclusion

Summary

Traduction automatique

Traduction automatique

Membrane trafficking involves transport of proteins from the plasma membrane to the cell interior (i.e. endocytosis) followed by trafficking to lysosomes for degradation or to the plasma membrane for recycling. Methods described in this article are designed to study endocytosis and recycling of plasma membrane proteins.

Tags

Keywords: Cell-based L-glutathione Protection AssayEndocytosisRecyclingPlasma Membrane ProteinsMembrane TraffickingBiotinylationStreptavidin AgaroseWestern Blotting

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