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Methods for the Modulation and Analysis of NF-κB-dependent Adult Neurogenesis
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Neurosciences
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Journal JoVE Neurosciences
Methods for the Modulation and Analysis of NF-κB-dependent Adult Neurogenesis

Methods for the Modulation and Analysis of NF-κB-dependent Adult Neurogenesis

DOI:
10.3791/50870-v

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14:58 min

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February 13, 2014

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, , , , ,
1Cell Biology,University of Bielefeld, 2Molecular Neurobiology,University of Bielefeld

Chapitres

  • 00:05Titre
  • 02:19Preparing the Spatial Pattern Separation-Barnes Maze
  • 04:27Running the Spatial Pattern Separation-Barnes Maze
  • 06:15Sectioning the Perfused Animals and Investing the Mossy Fiber Projections
  • 08:41Neurodegeneration and Behavioral Defects from IκBα Expression via CAMKIIα Promoter Control
  • 14:22Conclusion

Summary

Traduction automatique

Traduction automatique

Methods for the manipulation and analysis of NF-κB-dependent adult hippocampal neurogenesis are described. A detailed protocol is presented for a dentate gyrus-dependent behavioral test (termed the spatial pattern separation-Barnes maze) for the investigation of cognitive outcome in mice. This technique should also help enable investigations in other experimental settings.

Tags

NF-κBAdult NeurogenesisHippocampusDentate GyrusSpatial Pattern SeparationBarnes MazeTransgenic Mouse ModelNeuronal Plasticity

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