Journal
/
Médecine
/
Coculture System with an Organotypic Brain Slice and 3D Spheroid of Carcinoma Cells
Journal JoVE
Médecine
This content is Free Access.
Journal JoVE Médecine
Coculture System with an Organotypic Brain Slice and 3D Spheroid of Carcinoma Cells

Coculture System with an Organotypic Brain Slice and 3D Spheroid of Carcinoma Cells

DOI:
10.3791/50881-v

•

07:48 min

•

October 09, 2013

•

, , , , ,
1Department of Hematology and Oncology,University of Göttingen, 2Institute of Neuropathology,University of Göttingen, 3Institute of Anatomy and Cellbiology,University of Halle

Chapitres

  • 00:05Titre
  • 01:41Organotypic Brain Slice Coculturing
  • 04:11Immunofluorescence and Imaging of Astrocytes and Microglia
  • 05:42Representative Coculturing Results
  • 07:11Conclusion

Summary

Traduction automatique

Traduction automatique

The organotypic brain slice coculture with carcinoma cells enables visualizing morphological changes by fluorescence as well as bright field (video) microscopy during the process of carcinoma cell invasion of brain tissue. This model system also allows for cell exchange and replenishment approaches and offers a wide variety of manipulations and analyses.

Tags

CocultureOrganotypic Brain Slice3D SpheroidCarcinoma CellsCerebral MetastasisTumor MicroenvironmentTumor-associated Macrophages (TAM)AstrocytesIn Vitro ModelBrain ColonizationGlial ReactionsMolecular Analysis

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

Mammary Transplantation of Stromal Cells and Carcinoma Cells in C57BL/6J Mice

An Orthotopic Mouse Model of Anaplastic Thyroid Carcinoma

Assessment of Ovarian Cancer Spheroid Attachment and Invasion of Mesothelial Cells in Real Time

Three-Dimensional (3D) Tumor Spheroid Invasion Assay

An Organotypic High Throughput System for Characterization of Drug Sensitivity of Primary Multiple Myeloma Cells

A Brain Tumor/Organotypic Slice Co-culture System for Studying Tumor Microenvironment and Targeted Drug Therapies

A Cancer Cell Spheroid Assay to Assess Invasion in a 3D Setting

A Human Glioblastoma Organotypic Slice Culture Model for Study of Tumor Cell Migration and Patient-specific Effects of Anti-Invasive Drugs

Symmetric Bihemispheric Postmortem Brain Cutting to Study Healthy and Pathological Brain Conditions in Humans

Organotypic Slice Cultures as Preclinical Models of Tumor Microenvironment in Primary Pancreatic Cancer and Metastasis

Read Article