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与器官型脑片和癌细胞的3D球体共培养系统
Journal JoVE
Médecine
This content is Free Access.
Journal JoVE Médecine
Coculture System with an Organotypic Brain Slice and 3D Spheroid of Carcinoma Cells
DOI:

07:48 min

October 09, 2013

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Chapitres

  • 00:05Titre
  • 01:41Organotypic Brain Slice Coculturing
  • 04:11Immunofluorescence and Imaging of Astrocytes and Microglia
  • 05:42Representative Coculturing Results
  • 07:11Conclusion

Summary

Traduction automatique

在器官型脑切片的共培养与肿瘤细胞使肿瘤细胞侵袭的脑组织的过程中可视的形态变化用荧光以及明场(视频)显微镜。这个模型系统还允许细胞交换和补充的方法,并提供多种操作和分析。

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