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Use of pHluorin to Assess the Dynamics of Axon Guidance Receptors in Cell Culture and in the Chick Embryo
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Neurosciences
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Journal JoVE Neurosciences
Use of pHluorin to Assess the Dynamics of Axon Guidance Receptors in Cell Culture and in the Chick Embryo

Use of pHluorin to Assess the Dynamics of Axon Guidance Receptors in Cell Culture and in the Chick Embryo

DOI:
10.3791/50883-v

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10:30 min

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January 12, 2014

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, , ,
1University Claude Bernard Lyon1, CGphiMC UMR CNRS 5534,University of Lyon

Chapitres

  • 00:05Titre
  • 02:05Transfecting COS7 Cells
  • 05:26In ovo Electroporation of pHluorin-PlexinA1 Construct
  • 08:20Expression of pHluorin-PlexinA1
  • 09:26Conclusion

Summary

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Traduction automatique

We describe here the use of a pH-sensitive green fluorescent protein variant, pHluorin, to study the spatio-temporal dynamics of axon guidance receptors trafficking at the cell surface. The pHluorin-tagged receptor is expressed both in cell culture and in vivo, using electroporation of the chick embryo.

Tags

Keywords: PHluorinAxon Guidance ReceptorsCell CultureChick EmbryoSpatio-temporal DynamicsCell Surface DynamicsGrowth ConesSignaling MechanismsPH-dependent FluorescenceGFP VariantPlasma MembraneIn VitroIn VivoSpinal Cord

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