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Stretch in Brain Microvascular Endothelial Cells (cEND) as an In Vitro Traumatic Brain Injury Model of the Blood Brain Barrier
Journal JoVE
Médecine
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Journal JoVE Médecine
Stretch in Brain Microvascular Endothelial Cells (cEND) as an In Vitro Traumatic Brain Injury Model of the Blood Brain Barrier

Stretch in Brain Microvascular Endothelial Cells (cEND) as an In Vitro Traumatic Brain Injury Model of the Blood Brain Barrier

DOI:
10.3791/50928-v

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07:19 min

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October 26, 2013

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, ,
1Klinik und Poliklinik für Anästhesiologie,Zentrum für operative Medizin der Universität Würzburg, 2Department of Medicinal Chemistry, Faculty of Life Sciences,University of Vienna

Chapitres

  • 00:05Titre
  • 01:11Seeding of Endothelial Cells and Cell Differentiation
  • 02:50Stretch-induced Injury of Endothelial Cells
  • 03:46Assessment of Stretch Injury by Dye Uptake Assay and Lactate Dehydrogenase Release
  • 05:20Results: Stretch in Brain Microvascular Endothelial Cells as an In Vitro Traumatic Brain Injury Model of the Blood Brain Barrier
  • 06:50Conclusion

Summary

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In vitro traumatic brain injury models are being developed to reproduce in vivo brain deformation. Stretch-induced injury has been employed for astrocytes, neurons, glial cells, aortic, and brain endothelial cells. However, our system uses a blood brain barrier (BBB) model that possesses properties constituting a legitimate model of the BBB to establish an in vitro TBI model.

Tags

Traumatic Brain InjuryTBIIn Vitro ModelBrain Microvascular Endothelial CellsCENDBlood-brain BarrierBBBStretch InjuryPressure ApplicationCell PhysiologyIn Vivo TechniquesCell CultureExperimental Setup

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