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Mouse Genome Engineering Using Designer Nucleases
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Biologie
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Journal JoVE Biologie
Mouse Genome Engineering Using Designer Nucleases

Mouse Genome Engineering Using Designer Nucleases

DOI:
10.3791/50930-v

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12:04 min

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April 02, 2014

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, , ,
1Institute of Laboratory Animal Science,University of Zurich, 2Department of Genetics, Cell Biology & Development and Center for Genome Engineering,University of Minnesota

Chapitres

  • 00:05Titre
  • 01:18Identifying TALEN Target Sites
  • 02:30Embryo Isolation
  • 04:23Embryo Microinjection
  • 06:33Embryo Transfer
  • 09:15Identifying Founders with Targeted Mutations
  • 11:21Conclusion

Summary

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Traduction automatique

Designer nucleases such as zinc finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs) can be used to modify the genome of mouse preimplantation embryos by triggering both the nonhomologous end joining (NHEJ) and homologous recombination (HR) pathways. These advances enable the rapid generation of mice with precise genetic modifications.

Tags

Keywords: Mouse Genome EngineeringDesigner NucleasesZinc Finger Nucleases (ZFNs)Transcription Activator-like Effector Nucleases (TALENs)CRISPR/Cas9Site-specific Genome ModificationKnockoutKnock-inTransgenic MiceGenome EditingMicroinjectionFertilized Mouse Oocytes

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