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Mouse Genome Engineering Bruke Designer nukleaser
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Biologie
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Journal JoVE Biologie
Mouse Genome Engineering Using Designer Nucleases

Mouse Genome Engineering Bruke Designer nukleaser

DOI:
10.3791/50930-v

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12:04 min

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April 02, 2014

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, , ,
1Institute of Laboratory Animal Science,University of Zurich, 2Department of Genetics, Cell Biology & Development and Center for Genome Engineering,University of Minnesota

Chapitres

  • 00:05Titre
  • 01:18Identifying TALEN Target Sites
  • 02:30Embryo Isolation
  • 04:23Embryo Microinjection
  • 06:33Embryo Transfer
  • 09:15Identifying Founders with Targeted Mutations
  • 11:21Conclusion

Summary

Traduction automatique

Traduction automatique

Designer nukleaser som sink finger nukleaser (ZFNs) og transkripsjons aktivator lignende effektor nukleaser (Talens) kan brukes til å endre genomet muse preimplantation embryoer ved å utløse både ikkehomologe ende bli (NHEJ) og homolog rekombinasjon (HR) trasé. Disse fremskrittene muliggjøre rask generasjon av mus med presise genetiske modifikasjoner.

Tags

Keywords: Mouse Genome EngineeringDesigner NucleasesZinc Finger Nucleases (ZFNs)Transcription Activator-like Effector Nucleases (TALENs)CRISPR/Cas9Site-specific Genome ModificationKnockoutKnock-inTransgenic MiceGenome EditingMicroinjectionFertilized Mouse Oocytes

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