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Visualization of Endoplasmic Reticulum Subdomains in Cultured Cells
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Journal JoVE Biologie
Visualization of Endoplasmic Reticulum Subdomains in Cultured Cells

Visualization of Endoplasmic Reticulum Subdomains in Cultured Cells

DOI:
10.3791/50985-v

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16:43 min

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February 18, 2014

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, , ,
1Fondazione Filarete, 2Department of Biotechnology and Translational Medicine,University of Milan, 3Neuroscience Institute,National Research Council (CNR), 4Department of Health Science,“Magna Graecia” University of Catanzaro

Chapitres

  • 00:05Titre
  • 01:37Plasmid, Cell Culture, and Transfection with ER Fluorescent Proteins
  • 03:21Live Fluorescence Scanning Confocal Microscopy
  • 08:35Ultrastructural Analysis by Means of Transmission Electron Microscopy
  • 12:38Representative Imaging Results
  • 16:04Conclusion

Summary

Traduction automatique

Traduction automatique

We describe the imaging approaches we use to investigate the distribution and mobility of the transfected fluorescent proteins resident in the endoplasmic reticulum (ER) by means of the confocal imaging of living cells. We also ultrastructurally analyze the effect of their expression on the architecture of this subcellular compartment.

Tags

Endoplasmic ReticulumCell CompartmentsProtein TraffickingLipid MobilityFRAPFLIPLive-cell ImagingFluorescence MicroscopyElectron MicroscopyOrganelle SubcompartmentsPhysiological ConditionsPathological Conditions

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