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Using Microfluidics Chips for Live Imaging and Study of Injury Responses in Drosophila Larvae
This content is Free Access.
1Department of Molecular, Cellular and Developmental Biology,University of Michigan, 2Department of Biomedical Engineering,University of Michigan, 3Life Sciences Institute,University of Michigan, 4Department of Cell and Developmental Biology,University of Michigan, 5Department of Mechanical Engineering,University of Michigan
Chapitres
- 00:05Titre
- 01:47Preparing the PDMS Chip
- 03:47Using the Larva Chip for Live Imaging
- 07:23Inducing a Nerve Crush Injury to Larval Segmental Nerves
- 08:42Immobilization Enhanced Imaging
- 10:25Conclusion
Drosophila larvae are an attractive model system for live imaging due to their translucent cuticle and powerful genetics. This protocol describes how to utilize a single-layer PDMS device, called the 'larva chip' for live imaging of cellular processes within neurons of 3rd instar Drosophila larvae.
