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A Novel Light Damage Paradigm for Use in Retinal Regeneration Studies in Adult Zebrafish
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Journal JoVE Neurosciences
A Novel Light Damage Paradigm for Use in Retinal Regeneration Studies in Adult Zebrafish

A Novel Light Damage Paradigm for Use in Retinal Regeneration Studies in Adult Zebrafish

DOI:
10.3791/51017-v

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09:31 min

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October 24, 2013

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,
1Department of Anatomy and Cell Biology,Wayne State University School of Medicine, 2Department of Opthalmology,Wayne State University School of Medicine

Chapitres

  • 00:05Titre
  • 01:27UV Light Exposure
  • 03:28Halogen Lamp Light Exposure
  • 05:09Tissue Collection
  • 07:05Results: A Novel Light Damage Paradigm for Use in Retinal Regeneration Studies in Adult Zebrafish
  • 09:02Conclusion

Summary

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Traduction automatique

Multiple light damage protocols have been described to damage photoreceptors and consequently induce a retinal regeneration response in adult zebrafish. This protocol describes an improved method that can be used in pigmented animals and that damages the vast majority of rod and cone photoreceptors across the entire retina.

Tags

Light-induced Retinal DegenerationLIRD ParadigmPhotoreceptor DegenerationMüller Glial CellsRetinal RegenerationAdult ZebrafishRod And Cone PhotoreceptorsTransient-amplifying ProgenitorsPigmented Animals

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