Journal
/
Bio-ingénierie
/
Imaging Denatureret Kollagen Strands<em> In vivo</em> Og<em> Ex vivo</em> Via Photo-udløst Hybridisering af Caged Collagen mimetiske peptider
Journal JoVE
Bio-ingénierie
This content is Free Access.
Journal JoVE Bio-ingénierie
Imaging Denatured Collagen Strands In vivo and Ex vivo via Photo-triggered Hybridization of Caged Collagen Mimetic Peptides

Imaging Denatureret Kollagen Strands<em> In vivo</em> Og<em> Ex vivo</em> Via Photo-udløst Hybridisering af Caged Collagen mimetiske peptider

DOI:
10.3791/51052-v

•

07:03 min

•

January 31, 2014

•

, , ,
1Department of Bioengineering,University of Utah, 2Department of Radiology and Radiological Science,Johns Hopkins University School of Medicine, 3Institute for NanoBiotechnology,Johns Hopkins University

Chapitres

  • 00:05Titre
  • 01:25Photo-activation and IV Injection of Caged Collagen Mimetic Peptides (CMPs)
  • 02:22Near Infrared (NIR) Fluorescence Imaging of Skeletal Collagen Remodeling Activity In vivo
  • 03:31Visualization of Collagens in Ex vivo Tissue Sections
  • 05:19Representative Images
  • 06:28Conclusion

Summary

Traduction automatique

Traduction automatique

Denne procedure in vivo nær-IR fluorescensimagografi af kollagen remodeling aktiviteter i mus såvel som ex vivo-farvning af collagener i vævssnit ved hjælp af bur kollagen mimetiske peptider, der kan være tilgængeligt udløses til at hybridisere med denaturerede kollagen strenge viser.

Tags

CollagenDenatured CollagenExtracellular MatrixCaged Collagen Mimetic PeptidePhoto-triggered HybridizationIn Vivo ImagingEx Vivo ImagingProteasesTriple Helix

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

High Resolution 3D Imaging of Ex-Vivo Biological Samples by Micro CT

Ex vivo Mimicry for normale og unormale menneskelige hematopoiese

Virale Nanopartikler for<em> In vivo</em> Tumor Imaging

In vitro Syntese af Native, Fibrøst Long Mellemrum og Segmental Long Spacing Collagen

Analyse af Cell Migration inden for en tre-dimensionel kollagenmatrix

Engineering 3D Cellularized kollagengeler for Vascular Tissue Regeneration

Udviklet 3D Silke-collagen-baserede model for polariseret nervevæv

Layer-by-lags kollagenaflejring i mikrofluidapparater for Microtissue Stabilisering

Udarbejdelse af 3D Kollagen Gels og mikrokanalerne for Studiet af 3D Interactions<em> In vivo</em

Kvantificering af Fibrillar Kollagen organisation med Curvelet Transform-baserede værktøjer

Read Article