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ניתוח של אפופטוזיס בעוברי דג הזברה על ידי כל הר Immunofluorescence לזיהוי caspase הופעל 3
Journal JoVE
Biologie
This content is Free Access.
Journal JoVE Biologie
Analysis of Apoptosis in Zebrafish Embryos by Whole-mount Immunofluorescence to Detect Activated Caspase 3
DOI:

08:20 min

December 20, 2013

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Chapitres

  • 00:05Titre
  • 01:24Preparation of Solutions and Embryos
  • 03:17Immunofluorescence Staining and Microscopy
  • 05:43Representative Results
  • 07:56Conclusion

Summary

Traduction automatique

Certain genetic perturbations or exposure to toxins can disrupt normal developmental processes leading to death of specific cell types. The analysis of activated Caspase 3 by whole-mount immunofluorescence in zebrafish embryos reveals stage- and tissue-specific localization of cells specifically undergoing apoptosis.

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