Journal
/
Biologie
/
Omvendt Gær To hybrid system til at identificere Mammalian Nuclear receptor Rester at interagere med ligander og / eller-antagonister
Journal JoVE
Biologie
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Biologie
Reverse Yeast Two-hybrid System to Identify Mammalian Nuclear Receptor Residues that Interact with Ligands and/or Antagonists

Omvendt Gær To hybrid system til at identificere Mammalian Nuclear receptor Rester at interagere med ligander og / eller-antagonister

DOI:
10.3791/51085-v

•

10:51 min

•

November 15, 2013

•

, , ,
1Department of Genetics,Albert Einstein College of Medicine , 2Shanghai Key Laboratory of Complex Prescription and MOE Key Laboratory for Standardization of Chinese Medicines, Institute of Chinese Materia Medica,Shanghai University of Traditional Chinese Medicine

Chapitres

  • 00:05Titre
  • 02:47Construction of PXR and SRC-1 Fusions in Yeast Vectors
  • 03:49Yeast Two-hybrid Assays
  • 05:26X-gal Filter Assay
  • 06:32Liquid β-gal Assay
  • 07:49Results: X-gal Lift and β-gal Liquid Assay Interpretation
  • 09:06Conclusion

Summary

Traduction automatique

Traduction automatique

Ketoconazole binds to and antagonizes Pregnane X Receptor (PXR) activation. Yeast high throughput screens of PXR mutants define a unique region for ketoconazole binding. This yeast-based genetic method discovers novel nuclear receptor interactions with ligands that associate with surface binding sites.

Tags

Reverse Yeast Two-hybrid SystemNuclear ReceptorLigandsAntagonistsPregnane X Receptor (PXR)KetoconazoleCoactivator SRC-1Genetic AssayAntagonist Binding Sites

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/51085?article_type=v

Proteomics to Identify Proteins Interacting with P2X2 Ligand-Gated Cation Channels
check_url/fr/51085?article_type=v

Split-Ubiquitin Based Membrane Yeast Two-Hybrid (MYTH) System: A Powerful Tool For Identifying Protein-Protein Interactions
check_url/fr/51085?article_type=v

Analysis of mRNA Nuclear Export Kinetics in Mammalian Cells by Microinjection
check_url/fr/51085?article_type=v

RNAi screening for at identificere Postembryonic fænotyper i C. elegans</em
check_url/fr/51085?article_type=v

Modificeret Gær-Two-Hybrid system til at identificere Proteiner Interaktion med Growth Factor Progranulin
check_url/fr/51085?article_type=v

Isolering af mRNA'er associeret med Gær Mitokondrier at studere Mekanismer af lokaliserede Oversættelse
check_url/fr/51085?article_type=v

siRNA Screening at identificere Ubiquitin og ubiquitinlignende System Regulators Biologisk Pathways i dyrkede pattedyrceller
check_url/fr/51085?article_type=v

Påvisning af østrogene ligander i produkter til personlig pleje ved hjælp af en gær østrogen skærm optimeret for Undergraduate undervisning laboratorium
check_url/fr/51085?article_type=v

En simpel fluorescens-baserede Reporter analyse til at identificere cellulære komponenter kræves for Ricin toksin en kæde (RTA) handel med gær
check_url/fr/51085?article_type=v

En Flow flowcytometri-baserede analyse til at identificere stoffer, der forstyrrer bindingen af Fluorescently-mærket CXC Chemokine Ligand 12 til CXC Chemokine Receptor 4

Read Article