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Automated Separation of C. elegans Variably Colonized by a Bacterial Pathogen
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Chapitres
- 00:05Titre
- 01:49Infection of Synchronized Young Adult Worms with Pseudomonas aeruginosa
- 04:44Setting up the Wormsorter
- 06:19Sorting Colonized vs Noncolonized Worms
- 08:28Results: Colonization is Not Required for the Transcriptional Response to P. aeruginosa
- 10:19Conclusion
The wormsorter facilitates genetic screens in Caenorhabditis elegans by sorting worms according to expression of fluorescent reporters. Here, we describe a new usage: sorting according to colonization by a GFP-expressing pathogen, and we employ it to examine the poorly understood role of pathogen recognition in initiating immune responses.
