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माउस cardiomyocytes के अलगाव और शारीरिक विश्लेषण
Journal JoVE
Biologie
Author Produced
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Journal JoVE Biologie
Isolation and Physiological Analysis of Mouse Cardiomyocytes
DOI:

11:02 min

September 07, 2014

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Chapitres

  • 00:00Titre
  • 01:20Introduction
  • 01:37Preparation of Reagents and Langendorff Apparatus
  • 03:11Mouse Preparation and Removal of Heart
  • 03:39Aorta Cannulation and Langendorf Perfusion
  • 05:25Myocyte Dissociation and Calcium Tolerance
  • 07:06Loading Cells with Fura-2
  • 08:00Microscope Setup
  • 09:01Measurement and Analysis of Contractility and Calcium Transients
  • 10:39Conclusion

Summary

Traduction automatique

Individual cardiomyocytes from wild type and mutant mice can be isolated from the heart in order to study their contractility and calcium transients. This allows characterization of the contribution of cellular dysfunction to heart dysfunction from any cause.

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