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Multilayer Mounting for Long-term Light Sheet Microscopy of Zebrafish
Journal JoVE
Biologie
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Journal JoVE Biologie
Multilayer Mounting for Long-term Light Sheet Microscopy of Zebrafish

Multilayer Mounting for Long-term Light Sheet Microscopy of Zebrafish

DOI:
10.3791/51119-v

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07:28 min

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February 27, 2014

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, ,
1Huisken Lab,Max Planck Institute of Molecular Cell Biology and Genetics

Chapitres

  • 00:05Titre
  • 01:55Preparation of Fluorinated Ethylene Propylene Tubes
  • 03:02Preparation of Zebrafish and Multilayer Mounting
  • 06:26Results: Zebrafish Multi-day Imaging
  • 06:59Conclusion

Summary

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Traduction automatique

The development of zebrafish can be followed over days with light sheet microscopy when embryos are embedded in optically clear polymer tubes with low-concentration agarose.

Tags

Light Sheet MicroscopyZebrafishEmbryonic DevelopmentPhoto-toxicityBleachingLong-term Time-lapse ImagingSample MountingAgaroseOptically Clear TubesReal-time Developmental Biology

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