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Immunologie et infection
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Methodology for the Efficient Generation of Fluorescently Tagged Vaccinia Virus Proteins
Journal JoVE
Immunologie et infection
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Journal JoVE Immunologie et infection
Methodology for the Efficient Generation of Fluorescently Tagged Vaccinia Virus Proteins

Methodology for the Efficient Generation of Fluorescently Tagged Vaccinia Virus Proteins

DOI:
10.3791/51151-v

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09:27 min

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January 17, 2014

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, , , , ,
1School of Molecular Bioscience,University of Sydney, 2Department of Medicine,Center for Vascular Research, 3Asia Pacific Centre for Animal Health, Faculty of Veterinary Science,University of Melbourne

Chapitres

  • 00:05Titre
  • 01:34Creating the Recombinant Vector
  • 02:48Recombinant Virus Generation
  • 05:26Plaque Assay: NO Selection
  • 05:59Purity of Viral Stocks: PCR Screening of Genomic DNA
  • 07:23Results: Recombinant Vectors and the VACV Genome
  • 08:43Conclusion

Summary

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Traduction automatique

A rapid and modular protocol for the generation of recombinant vaccinia viruses expressing fluorescently tagged proteins simultaneously using the method of transient dominant selection is described here.

Tags

Keywords: Fluorescent Protein TaggingVaccinia VirusRecombinant Virus GenerationHomologous RecombinationTransient Dominant SelectionMetabolic SelectionLive-cell MicroscopyVirus-host Interactions

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