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疟疾蚊子中的二维和三维染色体绘画
Journal JoVE
Immunologie et infection
This content is Free Access.
Journal JoVE Immunologie et infection
2D and 3D Chromosome Painting in Malaria Mosquitoes
DOI:

09:57 min

January 06, 2014

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Chapitres

  • 00:05Titre
  • 01:11Overview of Isolating DNA from Polytene Chromosomes by Laser Capture Microdissection
  • 02:07Probe Preparation via Whole Genome Amplification
  • 03:593D FISH on Whole-mount Ovarian Tissue
  • 07:33WGA Kit Yields and FISH Labeling
  • 09:31Conclusion

Summary

Traduction automatique

染色体绘图是研究细胞核组织与卡约型演化的有用方法。在这里,我们演示了一种方法,将特定感兴趣的区域从单聚酯染色体中分离和放大,这些染色体随后用于 原位 杂交 (FISH) 的二维和三维荧光。

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