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ECM Protein Nanofibers and Nanostructures Engineered Using Surface-initiated Assembly
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Journal JoVE Bio-ingénierie
ECM Protein Nanofibers and Nanostructures Engineered Using Surface-initiated Assembly

ECM Protein Nanofibers and Nanostructures Engineered Using Surface-initiated Assembly

DOI:
10.3791/51176-v

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16:33 min

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April 17, 2014

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, ,
1Department of Biomedical Engineering,Carnegie Mellon University, 2Department of Materials Science and Engineering,Carnegie Mellon University

Chapitres

  • 00:05Titre
  • 03:06Making the PDMS Stamps
  • 04:35Microcontact Printing of ECM Patterns
  • 07:58Release of ECM Nanofibers and Nanostructures
  • 09:48Results: SIA Engineers ECM Protein Nanofibers with Precise Control Over Fiber Dimensions
  • 15:23Conclusion

Summary

Traduction automatique

Traduction automatique

A method to obtain nanofibers and complex nanostructures from single or multiple extracellular matrix proteins is described. This method uses protein-surface interactions to create free-standing protein-based materials with tunable composition and architecture for use in a variety of tissue engineering and biotechnology applications.

Tags

Extracellular Matrix (ECM)ECM Protein NanofibersECM NanostructuresSurface-initiated Assembly (SIA)Fibronectin FibrillogenesisIntegrin ReceptorsActo-myosinMicrocontact PrintingPoly(N-isopropylacrylamide) (PIPAAm)LamininFibrinogenCollagen

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